Voyager DP-263-S Guia do Utilizador descarregar pdf (Página 377)

100

101

Acquiring Spectra from the Sequence Control Panel

Voyager

™

Biospectrometry

™

Workstation User’s Guide 6-65

6.7.2 Before Creating a Sequence

This section describes:

• Optimizing instrument settings (.BIC) files for a

sequence run

• Creating macros

• Creating calibration (.CAL) files

• Creating settings (.SET) files

Optimizing

instrument

settings (.BIC)

files for a

sequence run

Select .BIC files based on the compound type and mass range

you are analyzing. See Section 5.1.2, Standard Instrument

Settings (.BIC) Files Provided.

You can use more than one .BIC file in your sequence run if

you are analyzing a wide mass range, for example, when

analyzing a mixture of protein and peptide samples. As a

general guideline, you may need separate .BIC files for

different mass ranges, for example:

• 1,000 to 6,000 Da

• 5,000 to 12,000 Da

• 10,000 to 30,000 Da

.BIC requirements

Optimize the instrument settings for Automatic Control mode.

For more information see Section 6.6.2, Setting Instrument

Settings for Automatic Control Mode.

Note the following:

• If Use Automated Laser Intensity Adjustment is disabled

in the .BIC, the laser intensity set in the Instrument

Control Panel is used for the analysis. You can manually

adjust the laser intensity by pressing Ctrl+PageUp or

Ctrl+PageDn.

• If Use Automated Sample Positioning is disabled in the

.BIC, the analysis is performed on the center of the

sample position. You can manually adjust the position

using the control stick.

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